Comparison of Western blot and ELISA to detect the Antibody Titers in Human Toxoplasmosis

Abstract

Toxoplasmosis is a zoonotic parasitic disease which is caused by Toxoplasma gondii that infects all warm-blooded mammals including human beings. In the current study, it was explored to Toxoplasma-specific IgG titer while comparing quantitative ELISA and WB. Ten human sera samples labelled as 53, 217, 28, 277, 229, 07, 204, 275, 241 and 04, (known positive as a result of earlier testing in a commercial ELISA kit. were analysed through quantitative ELISA and WB. The WB analysis revealed antibody titers ranging from 800 to12800 with one sample (28) possessing a titer of 1:800, two samples (07, 241) at 1:1600, five samples (53, 277, 204, 275, 04) at 1:3200, one sample (217) at 1:6400 and one sample (229) at 1:12800. The same dilutions of these samples were subjected to our indigenous quantitative ELISA (coated with recombinant SAG1) to determine and antibody (IgG) titer that ranged from 200 to 1600 with two samples (28, 277) at a titer of 1:200, with four samples (53, 07, 204, 241) at a titer of 1:400, with three samples (217, 275, 04) at a titer of 1:800 and with one sample (229) at a titer of 1:1600. In our study, the titers of T. gondii-specific-IgG antibodies revealed in WB were found much higher as compared to those in ELISA but there is a high positive correlation (rs = 0.714) analysed by spearman’s rho test