Determination of Adenosine phosphate in beef by QuEChERS- Graphene -UPLC

Author:

Duan Xiaoyan

Abstract

A rapid and highly sensitive modified QuEChERS method was combined with ultrahigh performance liquid chromatography (UPLC) to determine the content of adenosine phosphate in beef sample. In view of the complexity of the beef sample matrix, the cold perchloric acid was used as the solvent combined with vortex oscillation were selected to extracted the adenosine phosphate in the beef sample. 10mg graphene was used to remove interferences such as fat and pigment in beef, and UPLC was used for quantitative analysis. The three adenosine phosphates in beef showed a good linear relationship in the concentration range of 0.5-20 μg/mL, the linear correlation coefficient R2>0.99, and the average recovery rate of the three adenosine phosphate addition levels in beef samples was between 85.25%-100.12%, and the RSD was ranged from 1.63% to 3.69%. The adenosine phosphate content in commercially available beef samples further verifies the feasibility of this method. The proposed method has the advantages of simple and fast operation, good purification effect, high sensitivity and accuracy, therefor, may provide a new method for efficient and accurate evaluation of the quality and safety of beef

Publisher

Pakistan Journal of Agricultural Sciences

Subject

Plant Science,Soil Science,Agronomy and Crop Science,Food Science

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3