Reinitiation of DNA synthesis in senescent human fibroblasts upon fusion with cells of unlimited growth potential.

Author:

Norwood T H,Pendergrass W R,Martin G M

Abstract

Postreplicative, "senescent" human fibroblasts were fused to HeLa or to SV-40 transformed human fibroblasts with Sendai virus. DNA synthesis was reinitiated in senescent nuclei in a high proportion of the heterodikaryons. The [3H]thymidine labeling index of senescent fibroblast nuclei in heteropolykaryons was a function of the ratio of HeLa to senescent nuclei.

Publisher

Rockefeller University Press

Subject

Cell Biology

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