FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE-Reference-Cited by-同舟云学术

FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE

Published:1962-02-01 Issue:2 Volume:12 Page:385-410
ISSN:1540-8140
Container-title:Journal of Cell Biology
language:en
Short-container-title:

Author:

Palay Sanford L.¹,McGee-Russell S. M.¹,Gordon Spencer¹,Grillo Mary A.¹

Affiliation:

1. From the Laboratory of Neuroanatomical Sciences, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Maryland.

Abstract

This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.

Publisher

Rockefeller University Press

Subject

Cell Biology

Link

http://rupress.org/jcb/article-pdf/12/2/385/1064078/385.pdf

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