Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1-Reference-Cited by-同舟云学术

Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1

Published:2021-09-27 Issue:11 Volume:220 Page:
ISSN:0021-9525
Container-title:Journal of Cell Biology
language:en
Short-container-title:

Author:

Bessy Thomas¹²^ORCID,Candelas Adrian¹²^ORCID,Souquet Benoit¹²³^ORCID,Saadallah Khansa¹²^ORCID,Schaeffer Alexandre¹²^ORCID,Vianay Benoit¹²^ORCID,Cuvelier Damien⁴⁵⁶^ORCID,Gobaa Samy⁷,Nakid-Cordero Cecilia⁸^ORCID,Lion Julien⁸,Bories Jean-Christophe⁸,Mooney Nuala⁸^ORCID,Jaffredo Thierry⁹^ORCID,Larghero Jerome¹⁰,Blanchoin Laurent¹²^ORCID,Faivre Lionel¹⁰^ORCID,Brunet Stephane¹²^ORCID,Théry Manuel¹²^ORCID

Affiliation:

1. Cytomorpho Lab, Human Immunology, Pathophysiology, Immunotherapy, Unit 976, Institut National de la Santé et de la Recherche Médicale, CEA, Assistance Publique – Hôpitaux de Paris, Université de Paris, Institut de Recherche Saint Louis, Paris, France

2. Cytomorpho Lab, Laboratoire Physiologie Cellulaire et Végétale, UMR 5168, CEA, Institut national de recherche en agriculture, alimentation et environment, Centre national de la recherche scientifique, Université Grenoble-Alpes, Interdisciplinary Research Institute of Grenoble, Grenoble, France

3. Alveole, Paris, France

4. Sorbonne Université, Paris, France

5. Institut Pierre Gilles de Gennes, Paris Sciences et Lettres Research University, Paris, France

6. Institut Curie, Paris Sciences et Lettres Research University, Centre national de la recherche scientifique, UMR 144, Paris, France

7. Group of Biomaterials and Microfluidics Core Facility, Institut Pasteur, Paris, France

8. Human Immunology, Pathophysiology, Immunotherapy, Unit 976, Institut National de la Santé et de la Recherche Médicale, Université de Paris, Institut de Recherche Saint Louis, Paris, France

9. Laboratoire de Biologie du Développement, Centre national de la recherche scientifique, UMR 7622, Institut National de la Santé et de la Recherche Médicale U1156, Sorbonne Université, Institut de Biologie Paris-Seine, Paris, France.

10. Unité de Thérapie Cellulaire, Human Immunology, Pathophysiology, Immunotherapy, Unit 976, Assistance Publique – Hôpitaux de Paris, Hôpital Saint-Louis, Center of Clinical Investigations in Biotherapies of Cancer CBT501, Institut National de la Santé et de la Recherche Médicale, Université de Paris, Paris, France

Abstract

The fate of hematopoietic stem and progenitor cells (HSPCs) is regulated by their interaction with stromal cells in the bone marrow. However, the cellular mechanisms regulating HSPC interaction with these cells and their potential impact on HSPC polarity are still poorly understood. Here we evaluated the impact of cell–cell contacts with osteoblasts or endothelial cells on the polarity of HSPC. We found that an HSPC can form a discrete contact site that leads to the extensive polarization of its cytoskeleton architecture. Notably, the centrosome was located in proximity to the contact site. The capacity of HSPCs to polarize in contact with stromal cells of the bone marrow appeared to be specific, as it was not observed in primary lymphoid or myeloid cells or in HSPCs in contact with skin fibroblasts. The receptors ICAM, VCAM, and SDF1 were identified in the polarizing contact. Only SDF1 was independently capable of inducing the polarization of the centrosome–microtubule network.

Funder

Agence Nationale de la Recherche

European Research Council

Ville de Paris

Fondation Bettencourt Schueller

Fondation Schlumberger pour l’Education et la Recherche

Université de Paris

Ligue Contre le Cancer

Conseil Régional, Île-de-France

Canceropôle Ile-de-France

Association Saint-Louis

Association Jean-Bernard