The Hippo pathway polarizes the actin cytoskeleton during collective migration of Drosophila border cells

Author:

Lucas Eliana P.1,Khanal Ichha1,Gaspar Pedro1,Fletcher Georgina C.1,Polesello Cedric1,Tapon Nicolas1,Thompson Barry J.1

Affiliation:

1. Epithelial Biology Laboratory, and Apoptosis and Cell Proliferation Laboratory, Cancer Research UK, London Research Institute, London WC2A 3LY, England, UK

Abstract

Collective migration of Drosophila border cells depends on a dynamic actin cytoskeleton that is highly polarized such that it concentrates around the outer rim of the migrating cluster of cells. How the actin cytoskeleton becomes polarized in these cells to enable collective movement remains unknown. Here we show that the Hippo signaling pathway links determinants of cell polarity to polarization of the actin cytoskeleton in border cells. Upstream Hippo pathway components localize to contacts between border cells inside the cluster and signal through the Hippo and Warts kinases to polarize actin and promote border cell migration. Phosphorylation of the transcriptional coactivator Yorkie (Yki)/YAP by Warts does not mediate the function of this pathway in promoting border cell migration, but rather provides negative feedback to limit the speed of migration. Instead, Warts phosphorylates and inhibits the actin regulator Ena to activate F-actin Capping protein activity on inner membranes and thereby restricts F-actin polymerization mainly to the outer rim of the migrating cluster.

Publisher

Rockefeller University Press

Subject

Cell Biology

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