ISOLATION OF RIBOSOMES FROM CYSTS OF ENTAMOEBA INVADENS

Author:

Morgan Richard S.1,Slayter Henry S.1,Weller David L.1

Affiliation:

1. From the Children's Cancer Research Foundation, Boston, Massachusetts 02115.

Abstract

A reproducible method for the preparation of 75S ribosomes from cysts of Entamoeba invadens is presented. The method depends on the inactivation of the cyst's ribonuclease by high levels of Bentonite. The ribosomes are found to be extremely sensitive to ribonuclease, and to be stabilized by the addition of manganous and calcium ions to the magnesium customarily employed. Reasons are given for equating these ribosomes with the particles of which the crystalline chromatoid bodies are made.

Publisher

Rockefeller University Press

Subject

Cell Biology

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1. Acanthamoeba castellanii cysts: new ultrastructural findings;Parasitology Research;2013-01-15

2. Amplification of ribosomal DNA in Acheta;Hereditas;2009-02-12

3. Actin mRNA Levels and Actin Synthesis During the Encystation of Entamoeba invadens;The Journal of Eukaryotic Microbiology;1994-07

4. Parasitic Amebas of the Intestinal Tract;Parasitic Protozoa;1993

5. Nucleic Acids ofEntamoeba histolytica;The Journal of Protozoology;1989-03

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