ALTERATION OF THE CONFORMATION OF PROTEINS IN RED BLOOD CELL MEMBRANES AND IN SOLUTION BY FIXATIVES USED IN ELECTRON MICROSCOPY

Author:

Lenard John1,Singer S. J.1

Affiliation:

1. From the Department of Biology, University of California at San Diego, La Jolla, California 92037.

Abstract

The effects of several commonly employed fixatives on the three-dimensional conformations of two soluble proteins and the protein of intact red blood cell membranes have been studied by means of circular dichroism measurements in the spectral region of the peptide absorption bands. The fixatives used produced significant and parallel conformational changes in all of the proteins, in the increasing order: glutaraldehyde; OsO4; glutaraldehyde followed by OsO4; and KMnO4. The last two treatments obliterated most of the helical character of the proteins. The significance of these observations to the preparation of specimens for electron microscopy is discussed.

Publisher

Rockefeller University Press

Subject

Cell Biology

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