TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse

Author:

Na Bo-Ra1,Kim Hye-Ran1,Piragyte Indre1,Oh Hyun-Mee2,Kwon Min-Sung1,Akber Uroos1,Lee Hyun-Su1,Park Do-Sim3,Song Woo Keun1,Park Zee-Yong1,Im Sin-Hyeog34,Rho Mun-Chual5,Hyun Young-Min6,Kim Minsoo6,Jun Chang-Duk1

Affiliation:

1. School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea

2. Bioindustrial Process Research Center, Korea Research Institute Bioscience and Biotechnology (KRIBB), Jeongeup 580-185, South Korea

3. Academy of Immunology and Microbiology (AIM), Institute for Basic Science (IBS), Pohang 790-784, South Korea

4. Division of Integrative Biosciences and Biotechnology (IBB), Pohang University of Science and Technology, Pohang 790-784, South Korea

5. Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan 570-749, South Korea

6. Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY 14642

Abstract

The formation of an immunological synapse (IS) requires tight regulation of actin dynamics by many actin polymerizing/depolymerizing proteins. However, the significance of actin stabilization at the IS remains largely unknown. In this paper, we identify a novel function of TAGLN2—an actin-binding protein predominantly expressed in T cells—in stabilizing cortical F-actin, thereby maintaining F-actin contents at the IS and acquiring LFA-1 (leukocyte function-associated antigen-1) activation after T cell receptor stimulation. TAGLN2 blocks actin depolymerization and competes with cofilin both in vitro and in vivo. Knockout of TAGLN2 (TAGLN2−/−) reduced F-actin content and destabilized F-actin ring formation, resulting in decreased cell adhesion and spreading. TAGLN2−/− T cells displayed weakened cytokine production and cytotoxic effector function. These findings reveal a novel function of TAGLN2 in enhancing T cell responses by controlling actin stability at the IS.

Publisher

Rockefeller University Press

Subject

Cell Biology

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