Affiliation:
1. From the Department of Pathology (Division of Neuropathology), University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 and the Laboratory of Immunocytochemistry, Pasteur Institute, Paris, France
Abstract
With the use of the cytochemical stain for horseradish peroxidase of Graham and Karnovsky (1966. J. Histochem. Cytochem. 14:291), conjugates of horseradish peroxidase with ricin, wheat germ agglutinin, and phytohemagglutinin were employed for the morphologic demonstration of d-galactose (ricin), N-acetylglucosamine (wheat germ), and N-acetylgalactosamine (phytohemagglutinin) containing moieties on the surface of unfixed, or paraformaldehyde-fixed rat lymphoid cells. D-Galactose, or d-galactose containing disaccharides inhibited the interaction between ricin peroxidase and lymphoid cell surface; also, N-acetylglucosamine inhibited the wheat germ peroxidase-lymphoid cell interaction, but N-acetylgalactosamine failed to inhibit the reaction between phytohemagglutinin peroxidase and the surface of lymphoid cells.
Publisher
Rockefeller University Press
Cited by
96 articles.
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