The Cytokinesis Gene KEULE Encodes a Sec1 Protein That Binds the Syntaxin Knolle

Author:

Assaad Farhah F.1,Huet Yoann2,Mayer Ulrike3,Jürgens Gerd3

Affiliation:

1. Genetics and Microbiology Institute, Ludwig Maximilians University, D-80638 Munich, Germany

2. Plant Science Institute, ETH-Zürich, CH 8092 Zürich, Switzerland

3. Department of Developmental Genetics, University of Tübingen, D-72076 Tübingen, Germany

Abstract

KEULE is required for cytokinesis in Arabidopsis thaliana. We have positionally cloned the KEULE gene and shown that it encodes a Sec1 protein. KEULE is expressed throughout the plant, yet appears enriched in dividing tissues. Cytokinesis-defective mutant sectors were observed in all somatic tissues upon transformation of wild-type plants with a KEULE–green fluorescent protein gene fusion, suggesting that KEULE is required not only during embryogenesis, but at all stages of the plant's life cycle. KEULE is characteristic of a Sec1 protein in that it appears to exist in two forms: soluble or peripherally associated with membranes. More importantly, KEULE binds the cytokinesis-specific syntaxin KNOLLE. Sec1 proteins are key regulators of vesicle trafficking, capable of integrating a large number of intra- and/or intercellular signals. As a cytokinesis-related Sec1 protein, KEULE appears to represent a novel link between cell cycle progression and the membrane fusion apparatus.

Publisher

Rockefeller University Press

Subject

Cell Biology

Reference32 articles.

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3. Cytokinesis in somatic plant cells;Assaad;Plant Physiol. Biochem,1997

4. An Arabidopsis VPS45 homolog implicated in protein transport to the vacuole;Bassham;Plant Physiol,1998

5. Syntaxin 6 functions in trans-Golgi network vesicle trafficking;Bock;Mol. Biol. Cell,1997

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