VASH1–SVBP and VASH2–SVBP generate different detyrosination profiles on microtubules

Author:

Ramirez-Rios Sacnicte1ORCID,Choi Sung Ryul2ORCID,Sanyal Chadni1ORCID,Blum Thorsten B.2ORCID,Bosc Christophe1ORCID,Krichen Fatma1ORCID,Denarier Eric1ORCID,Soleilhac Jean-Marc1ORCID,Blot Béatrice1ORCID,Janke Carsten34ORCID,Stoppin-Mellet Virginie1ORCID,Magiera Maria M.34ORCID,Arnal Isabelle1ORCID,Steinmetz Michel O.25ORCID,Moutin Marie-Jo1ORCID

Affiliation:

1. Univ. Grenoble Alpes, Inserm, U1216, Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Grenoble Institut Neurosciences, Grenoble, France 1

2. Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland 2

3. Institut Curie, Université Paris Sciences et Lettres, Centre National de la Recherche Scientifique UMR3348, Orsay, France 3

4. Université Paris-Saclay, Centre National de la Recherche Scientifique UMR3348, Orsay, France 4

5. Biozentrum, University of Basel, Basel, Switzerland 5

Abstract

The detyrosination/tyrosination cycle of α-tubulin is critical for proper cell functioning. VASH1–SVBP and VASH2–SVBP are ubiquitous enzymes involved in microtubule detyrosination, whose mode of action is little known. Here, we show in reconstituted systems and cells that VASH1–SVBP and VASH2–SVBP drive the global and local detyrosination of microtubules, respectively. We solved the cryo-electron microscopy structure of VASH2–SVBP bound to microtubules, revealing a different microtubule-binding configuration of its central catalytic region compared to VASH1–SVBP. We show that the divergent mode of detyrosination between the two enzymes is correlated with the microtubule-binding properties of their disordered N- and C-terminal regions. Specifically, the N-terminal region is responsible for a significantly longer residence time of VASH2–SVBP on microtubules compared to VASH1–SVBP. We suggest that this VASH region is critical for microtubule detachment and diffusion of VASH–SVBP enzymes on lattices. Our results suggest a mechanism by which VASH1–SVBP and VASH2–SVBP could generate distinct microtubule subpopulations and confined areas of detyrosinated lattices to drive various microtubule-based cellular functions.

Funder

Leducq Foundation

Agence National de la Recherche

Centre National de la Recherche Scientifique

Institut National de la Santé et de la Recherche Médicale

France Alzheimer

University Grenoble Alpes

Swiss National Science Foundation

Photonic Imaging Center of Grenoble Institute Neuroscience

Publisher

Rockefeller University Press

Subject

Cell Biology

Cited by 7 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3