Integration of cell wall synthesis and chromosome segregation during cell division in Caulobacter

Author:

Mahone Christopher R.1ORCID,Payne Isaac P.1ORCID,Lyu Zhixin2ORCID,McCausland Joshua W.2ORCID,Barrows Jordan M.1ORCID,Xiao Jie2ORCID,Yang Xinxing23ORCID,Goley Erin D.1ORCID

Affiliation:

1. Johns Hopkins University School of Medicine 1 Department of Biological Chemistry, , Baltimore, MD, USA

2. Johns Hopkins University School of Medicine 2 Department of Biophysics and Biophysical Chemistry, , Baltimore, MD, USA

3. MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, School of Basic Medical Sciences, University of Science and Technology of China 3 Division of Life Sciences and Medicine, , Hefei, China

Abstract

To divide, bacteria must synthesize their peptidoglycan (PG) cell wall, a protective meshwork that maintains cell shape. FtsZ, a tubulin homolog, dynamically assembles into a midcell band, recruiting division proteins, including the PG synthases FtsW and FtsI. FtsWI are activated to synthesize PG and drive constriction at the appropriate time and place. However, their activation pathway remains unresolved. In Caulobacter crescentus, FtsWI activity requires FzlA, an essential FtsZ-binding protein. Through time-lapse imaging and single-molecule tracking of Caulobacter FtsW and FzlA, we demonstrate that FzlA is a limiting constriction activation factor that signals to promote conversion of inactive FtsW to an active, slow-moving state. We find that FzlA interacts with the DNA translocase FtsK and place FtsK genetically in a pathway with FzlA and FtsWI. Misregulation of the FzlA-FtsK-FtsWI pathway leads to heightened DNA damage and cell death. We propose that FzlA integrates the FtsZ ring, chromosome segregation, and PG synthesis to ensure robust and timely constriction during Caulobacter division.

Funder

National Institutes of Health

National Natural Science Foundation of China

Anhui Provincial Natural Science Foundation

Publisher

Rockefeller University Press

Subject

Cell Biology

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