Affiliation:
1. From the Biological Laboratories, Harvard University, Cambridge
Abstract
Bacterial cells were fixed in OsO4, washed, dehydrated, and embedded in a methacrylate mixture. Ultrathin sections were cut on a Porter-Blum ultramicrotome and were examined in an RCA electron microscope, type EMU-2D.
The sections revealed that the cytoplasmic membrane undergoes a centripetal growth to form a membrane septum. This septum is formed as a double structure. Constriction of the daughter cells and deposition of cell wall material lead to the separation of the daughter cells.
The bacterial cytoplasm appears to consist largely of 200 A granules and occasionally reveals arrays of parallel dense lines.
Publisher
Rockefeller University Press
Cited by
50 articles.
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