Affiliation:
1. From the Department of Pharmacology and Institute of Biomedical Electronics, University of Toronto, Toronto, Canada
Abstract
Continuous free-flow electrophoretic separation has been used to obtain relatively pure preparations of synaptosomes and synaptic vesicles from crude fractions of guinea pig brain homogenates. Measurements of the contents of protein, neuraminic acid, and bound acetylcholine; the activities of succinic dehydrogenase, adenosine triphosphatase, choline acetylase, and 5'-nucleotidase; and the uptake of 14C-labeled choline arid acetylcholine in the presence and absence of hemicholinium, all confirm the electron microscope evidence that the electrophoretic preparations are at least as pure as those obtained by ultracentrifugal methods. The electrophoretic mobility measurements have been used to calculate zeta potentials and surface charge densities for these particles.
Publisher
Rockefeller University Press
Cited by
26 articles.
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1. Synaptosome as a tool in Alzheimer’s disease research;Brain Research;2020-11
2. Thirty years of synaptosome research;Journal of Neurocytology;1993-09
3. Praxis der präparativen Free-Flow-EIektrophorese;Praxis der elektrophoretischen Trennmethoden;1989
4. Membrane Fusion;Subcellular Biochemistry;1985
5. The Synaptosome;Structural Elements of the Nervous System;1984