LYSOZYME IN EPIPHYSEAL CARTILAGE-Reference-Cited by-同舟云学术

LYSOZYME IN EPIPHYSEAL CARTILAGE

Published:1971-05-01 Issue:2 Volume:49 Page:450-458
ISSN:1540-8140
Container-title:Journal of Cell Biology
language:en
Short-container-title:

Author:

Kuettner K. E.¹,Eisenstein R.¹,Soble L. W.¹,Arsenis C.¹

Affiliation:

1. From the Departments of Orthopaedic Surgery and Biochemistry and Division of Pathology, Rush Presbyterian-St. Luke's Medical Center, and Department of Biological Chemistry, University of Illinois College of Medicine, Chicago, Illinois 60612

Abstract

The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described.

Publisher

Rockefeller University Press

Subject

Cell Biology

Link

http://rupress.org/jcb/article-pdf/49/2/450/1385621/450.pdf

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