PP2ARts1 is a master regulator of pathways that control cell size

Author:

Zapata Jessica1,Dephoure Noah2,MacDonough Tracy1,Yu Yaxin3,Parnell Emily J.3,Mooring Meghan1,Gygi Steven P.2,Stillman David J.3,Kellogg Douglas R.1

Affiliation:

1. Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064

2. Department of Cell Biology, Harvard Medical School, Boston, MA 02115

3. Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84112

Abstract

Cell size checkpoints ensure that passage through G1 and mitosis occurs only when sufficient growth has occurred. The mechanisms by which these checkpoints work are largely unknown. PP2A associated with the Rts1 regulatory subunit (PP2ARts1) is required for cell size control in budding yeast, but the relevant targets are unknown. In this paper, we used quantitative proteome-wide mass spectrometry to identify proteins controlled by PP2ARts1. This revealed that PP2ARts1 controls the two key checkpoint pathways thought to regulate the cell cycle in response to cell growth. To investigate the role of PP2ARts1 in these pathways, we focused on the Ace2 transcription factor, which is thought to delay cell cycle entry by repressing transcription of the G1 cyclin CLN3. Diverse experiments suggest that PP2ARts1 promotes cell cycle entry by inhibiting the repressor functions of Ace2. We hypothesize that control of Ace2 by PP2ARts1 plays a role in mechanisms that link G1 cyclin accumulation to cell growth.

Publisher

Rockefeller University Press

Subject

Cell Biology

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