Affiliation:
1. From the Laboratory of Biochemistry, Section on Cellular Physiology, National Heart Institute, National Institutes of Health, Public Health Service, United States Department of Health, Education, and Welfare, Bethesda, Maryland 20014
Abstract
Thin films of methyl oleate, oleic acid, and di-octadecenoyl phosphatidylcholine were reacted with a 2% solution of OsO4 in water for 1 hr at 0°. As controls, methyl 9,10-dihydroxystearate and 9,10-dihydroxystearic acid were reacted with OsO4 in 0.25 N NaOH in methanol for 1 hr at room temperature. The reaction products were isolated, purified, and analyzed by thin-layer chromatography, gas-liquid chromatography, and infrared and visible spectroscopy. In all cases, the products were identified as diesters of osmic acid in which two molecules of fatty acids are linked through 1 molecule of osmic acid.
Publisher
Rockefeller University Press
Cited by
128 articles.
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