TURNOVER OF HELA RIBOSOMAL RNA

Author:

Nair C. N.1,Knight E.1

Affiliation:

1. From the Central Research Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898.

Abstract

A class of RNA in HeLa cytoplasm with a sedimentation value of 22S is described. This RNA is a true cytoplasmic component and is not an artifact of cell rupture or of the method of RNA preparation. The 22S RNA sediments in a sucrose gradient with those ribosomal structures containing 28S ribosomal RNA (60S and 74S particles and polyribosomes). It has a base composition and methyl content similar to those of 28S RNA. The kinetics of formation of 22S suggest that it is not a direct product of transcription but is derived in vivo from "old" molecules of 28S RNA.

Publisher

Rockefeller University Press

Subject

Cell Biology

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