FIRST DHA-PRODUCING KLEBSIELLA PNEUMONIAE ISOLATED FROM A URINARY TRACT INFECTION IN ROMANIA

Abstract

Beta-lactamase production is a major antibiotic resistance determinant in Enterobacterales. A carbapenemresistant Klebsiella pneumoniae isolate was recovered from routine screening in a tertiary urology hospital. The strain was evaluated phenotypically using the interpretative reading of an extended disk diffusion antibiogram and for specific antibiotics, the minimum inhibitory concentration (MIC) was performed. Phenotypic tests were further performed such as the CIM, mCIM, rCIM, the double disk synergy test, Blue Carba Test, and NGTEST® CARBA-5 and carbapenemase production was not confirmed. The isolate was sequenced and multiple resistance genes were identified. This report signifies the first description of DHA-1 in a Klebsiella pneumoniae strain in Romania.