Using Plant Cells of Nitellopsis obtusa for Biophysical Education-Reference-Cited by-同舟云学术

Using Plant Cells of Nitellopsis obtusa for Biophysical Education

Published:2020-11-24 Issue:1 Volume:2 Page:18-29
ISSN:2578-6970
Container-title:The Biophysicist
language:en
Short-container-title:

Author:

Pupkis Vilmantas¹^ORCID,Buisas Rokas¹,Lapeikaite Indre¹,Kisnieriene Vilma¹

Affiliation:

1. Department of Neurobiology and Biophysics, Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekio Avenue 7, LT-10257 Vilnius, Lithuania

Abstract

ABSTRACT Using giant characeaen algae Nitellopsis obtusa in laboratory exercises is proposed to familiarize students with basic concepts of electrophysiology and provide some simple hands-on practice. The described concept experiments present extracellular registration of action potentials (APs) and investigation of cytoplasmic streaming properties. Students are expected to register the propagation velocity of APs (found to be 3.4 ± 1.5 cm/s in N. obtusa), as well as the velocity of cytoplasmic streaming (66.7 ± 9 μm/s). Proposed exercises also concern recovery dynamics of cytoplasmic streaming after a stimulation (recovery time constant τ = 3.7 ± 2.1 min) as well as investigation of an effect of various chemicals (e.g., KCl) on all selected parameters. The experiments endorse characeaen algae as a model system to be routinely explored in education of biophysics and bioelectrical phenomena of the cell.

Publisher

Biophysical Society

Link

http://meridian.allenpress.com/the-biophysicist/article-pdf/2/1/18/2905127/i2578-6970-2-1-18.pdf

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1. Evolution of long-distance signalling upon plant terrestrialization: comparison of action potentials in Characean algae and liverworts;Annals of Botany;2022-08-01

2. Impact of Mammalian Two-Pore Channel Inhibitors on Long-Distance Electrical Signals in the Characean Macroalga Nitellopsis obtusa and the Early Terrestrial Liverwort Marchantia polymorpha;Plants;2021-03-29