Identification of Novel Gene Expression Patterns and Genetic Mechanisms in Asthma affected Patients Treated with Budesonide

Abstract

Aim: To identify the significant genes and pathways involved in asthma patients and asthma-affected patients treated with Budesonide is the aim of our research. Materials and Methods: DNA microarray analysis for asthma has been performed and significant DEGs are identified. Up-regulated genes and down-regulated genes were identified by GEO2R analysis. Gene-Gene interaction was predicted using the STRING. Gene Ontology was analyzed by using the STRING and FunRich. Hub genes were observed by using CytoHubba plugins of Cytoscape. Results: By analyzing GEO2R, 22 genes were upregulated genes and 16 genes were downregulated genes. We have obtained the gene comprising 28 nodes and 8 edges with an estimating clustering coefficient of 0.25 in pHBECs with not treated and pHBECs treated with Budesonide. Gene ontology has shown the 27 genes located in the large intestine as a COSMIC analysis more than other analyses. By using the CytoHubba plugin of Cytoscape, identified MMP3, TSLP, POSTN, ETS1, and SAA1 as hub genes. Conclusion: Due to this limitation, the medications that are brought into the market are not site-directed, and rather they showed random inhibitory actions. So we have developed a computational pipeline to identify the significant novel genes and novel pathways involved in the asthma patient and asthma-affected patient treated with Budesonide.