Generation of Human-induced Pluripotent Stem Cells (hiPSCs)-derived from Dermal Fibroblast of Schizophrenic Patients

Abstract

Introduction: Schizophrenia (SCZ) is a psychiatric disorder caused by environmental, social, and genetic factors. This phenomenon is a severe neuropsychiatric disorder with a 1% worldwide prevalence. As SCZ is an exclusively human disorder, animal models cannot mimic all SCZD pathophysiology. Thus, it is crucial to develop a novel human-based specific model of SCZD to elucidate mechanisms of the occurrence of the disease. In this regard, reprogramming somatic cells to human- induced pluripotent stem cells (hiPSCs) serve an expense instrument for modeling SCZ. Methods: In the present study, we directly reprogrammed the isolated human ear dermal fibroblasts (HDFs) from schizophrenic patients into hiPSCs using some episomal agents in matrigel coated plates. The existence of pluripotency markers was confirmed by the Immunocytochemistry (ICC) test and alkaline phosphatase protocol. We performed karyotype analysis to ensure the maintenance of the normal chromosomes. Results and Conclusion: Analysis of colonies exhibited intense alkaline phosphatase engagement and represent Oct4, SSEA4, Nanog, and Tra-1-60. HiPSCs show normal karyotype, and they were potent to differentiate to ectoderm, endoderm, and mesoderm. Application of hiPSCs derived from schizophrenic patients would be a promising approach to treating the disease. For checking the behavior of the cells during neurogenesis, we suggest further studies be applied.