COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS-Reference-Cited by-同舟云学术

COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS

Published:2014-11-03 Issue:2 Volume:20 Page:
ISSN:1900-1649
Container-title:Acta Biológica Colombiana
language:
Short-container-title:Acta biol. Colomb.

Author:

Muñoz Bodnar Alejandra,Cruz Gómez Leidy Mariel,Bernal Adriana,Szurek Boris,López Carrascal Camilo Ernesto

Abstract

Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB), a major disease for cassava crops in South America and Africa. Until now the development of the disease is measured via AUDPC (Area Under Disease Progress Curve) but no reliable quantitative methods are available probably due to high variability of bacterial growth in planta. To establish an accurate method for bacterial quantification during the course of Xam infection within the host tissues, we analyzed bacterial populations upon stem and leaf-puncturing as well as leaf-clipping of cassava varieties MCOL1522 and SG107-35 challenged with the virulent Xam strain CIO151. Here, we show that the movement of bacteria along the tissues and especially in leaves is stochastic. Moreover, we were able to demonstrate differential growth of virulent Xam strain CIO151 upon stem-puncturing and quantification of bacteria 6 cm. away from the inoculation point of two varieties displaying contrasting levels of susceptibility.RESUMENXanthomonas axonopodis pv. manihotis (Xam) es el agente causal del tizón bacteriano de la yuca, una de las principales enfermedades de los cultivos de yuca en América del Sur y África. Hasta ahora, el desarrollo de la enfermedad se mide a través de AUDPC (Area Under Disease Progress curve), pero no hay disponibles métodos cuantitativos fiables, esto debido posiblemente a la alta variabilidad del crecimiento bacteriano en la planta. Para establecer un método exacto para la cuantificación bacteriana durante el curso de la infección Xam dentro de los tejidos del huésped, se analizaron las poblaciones de bacterias sobre tallo y hojas, así como corte de hojas de las <a style="mso-comment-reference: as_1; mso-comment-date: 20141102T2035;">variedades de yuca</a>MCOL1522 y SG107-35 con la cepa virulenta CIO151 Xam. <a style="mso-comment-reference: as_2; mso-comment-date: 20141102T2035;">En esta investigación se </a>muestra que el movimiento de las bacterias a lo largo de los tejidos y especialmente en las hojas es estocástico. Por otra parte, hemos podido demostrar el crecimiento diferencial de la cepa virulenta Xam CIO151 tras la punción al tallo y la cuantificación de la bacteria a 6 cm de distancia del punto de inoculación de dos variedades que presentan niveles contrastantes de susceptibilidad.

Publisher

Universidad Nacional de Colombia

Subject

General Agricultural and Biological Sciences

Reference20 articles.

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