Acute and chronic effects of methanolic extract of Teucrium polium on blood parameters and histopathology of liver and kidney in Female Rats-Reference-Cited by-同舟云学术

Acute and chronic effects of methanolic extract of Teucrium polium on blood parameters and histopathology of liver and kidney in Female Rats

Published:2024-09-15 Issue:3 Volume:1 Page:1-12
ISSN:3020-7886
Container-title:Bionatura Journal
language:en
Short-container-title:BJ

Author:

Imane Krache¹^ORCID,Hayet Trabsa²^ORCID,Naouel Boussoualim¹^ORCID,Sorya Ouhida³^ORCID,Lekhmici Arrar¹^ORCID

Affiliation:

1. Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas of Setif 1, Setif 19000, Algeria

2. Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas of Setif 1, Setif 19000, Algeria, Faculties of Exact Sciencesand Nature and Life Sciences, University of Biskra, Biskra 07000

3. Laboratory of Anaphatology, Hospitalo-University Center (CHU) of Setif, Setif 19000, Algeria

Abstract

Teucrium polium L. is commonly used as a medicinal plant in Algeria to fight various human diseases. In this study, extracting aerial parts of T. polium with methanol gave a dry matter yield of 8.24 %. The effects of methanolic extract of T. polium "TPME" were examined per os on female rats Albino Wistar for six weeks. Biochemical and hematological serum parameters and morphology and histopathology of organs of treated rats were studied. Acute toxicity showed low toxicity with a lethal dose of 50% LD50 > 2400 mg / Kg of body weight. These data can be used to classify these plants as slightly toxic. However, sub-acute treatment for six weeks of rats with 75, 150, and 300 mg of methanolic extract / Kg of body weight significantly increased the most studied hematological parameters. Biochemical analysis revealed a significant increase of renal parameters (urea, creatinine, uric acid, Na, and K), accompanied by an increase in the relative weight of kidney, lipidic (cholesterol), and hepatic glutamate oxaloacetate transaminase (GOT) values in all treated rats. Histopathological examination confirmed the biochemical tests by the observation of perilobular necrosis areas, bile duct and inflammatory infiltration of the liver, and the presence of marked intracytoplasmic vacuoles in the kidney with the dose 300 mg of TPME Kg of animal body weight. Use of Teucrium polium L. may cause hepatotoxicity and nephrotoxicity after prolonged herb administration. Keywords: Teucrium polium L, TPME, LD50, biochemical parameters, toxicity

Publisher

Clinical Biotec

Reference31 articles.

1. 1. Meguellati H, OuafiS, SaadS, Djemouai N. Evaluation of acute, subacute oral toxicity and wound healing activity of mother plant and callus of Teucrium polium L. subsp. geyrii Maire from Algeria. S AFR J BOT. 2019; 127:25-34.

2. 2. Krache I, Boussoualim N, Charef N, Trabsa H, Ouhida S, Benbacha F, Daamouche ZE, Benzidane N, Baghiani A, Khennouf S, Arrar L.. Evaluation of acute and chronic toxic effects of Algerian ger-mander in Swiss albino mice. J App Pharm Sci. 2015; 5 (Suppl 3): 027-032.

3. 3. Noumi E, Snoussi M, Anouar E, Alreshidi M, Veettil V.N, Elkahoui S, Adnan M, Patel M, Kadri A, Aouadi K, De Feo V, Badraoui R . HR-LCMS-Based Metabolite Profiling, Antioxidant, and Anticancer Properties of Teucrium polium L. Methanolic Extract: Computational and In Vitro. Study Antioxidants .2020; 9: 1089; doi:10.3390 /antiox9111089.

4. 4. Özer Z, Kiliç T, Çarikçi S, Yilmaz H. Investigation of phenolic compounds and antioxidant activity of Teucrium polium L. decoction and infusion. BAUN Fen Bil. Enst. Dergisi. 2018;20(1) :212-218.

5. 5. Sharifi-Rad M, Pohl P, Epifano F, Zengin G, Jaradat N, Messaoudi M. Teucrium polium (L.): Phy-tochemical Screening and Biological Activities at Different.Phenological Stages. Molecules.2022; 27, 1561. https://doi.org/10.3390/ molecules27051561.