Effects of Valeriana officinalis and Ciprofloxacin on Kidney Histopathology in Rats Pyelonephritis by Pseudomonas aeruginosa

Abstract

Introduction: Urinary tract infection (UTI) is a bacterial infection that affects the urinary tract. The bacterial infection of the upper urinary tract is called nephritis. The purpose of this study was to evaluate the antibacterial effect of Valeriana officinalis and Ciprofloxacin on kidney histopathology in rats with pyelonephritis by Pseudomonas. Materials and Methods: In this study, 48 male Wistar rats were divided into six groups and each group had two replicates. The first group was considered a control and received 0.1 mg/ kg/day of saline daily for a month. The second group received a single injection of 0.5 McFarland of microbial suspension per kilogram of animal into the pelvis of the right kidney of the rats, resulting in pyelonephritis. The third group of rats received 0.9 mg/kg/day methanolic extract of Valeriana officinalis intraperitoneally for a month. The fourth group of rats received Ciprofloxacin intraperitoneally for a month at a dose of 0.6 mg/kg/day. Group five contained rats with pyelonephritis that received the antibiotic (Ciprofloxacin) at a dosage of 0.6 mg/kg/day. Group six with pyelonephritis received 0.9 mg/kg/day of Valeriana officinalis extract. The inflammation in the cortex, Pelvic, medulla, and tissue sections was studied at the end of the study. Results: The rats that received Valeriana officinalis extract improved pelvic and medullary tissue, the site of Pseudomonas bacteria, and prevented the destruction of renal cortex tissue. The rats that received Ciprofloxacin had fewer medullary and tissue inflammations. Conclusion: According to the results of this research, the extract not only improved the tissue of the pelvis and medulla, which is where Pseudomonas bacteria live, but it also inhibits the degradation of renal cortex tissue. It was proven to diminish medullary inflammation to some extent, but in rats, it exacerbated the loss of renal cortex tissue.