The Effect of Prolonged Incubation of Sperm at Testis Temperature versus Room Temperature on Semen Parameters- An Experimental Study

Author:

Ostadian Caspian,Mehrafza Marzieh,Eftekhari Azadeh,Aghajani Shahrzad,Vahabzadeh Hajar,Gholami Mahdis,Raoufi Azadeh,Samadnia Sajedeh,Hosseinzadeh Elmira,Hosseini Ahmad

Publisher

Farname, Inc.

Subject

Obstetrics and Gynecology,Oncology,Embryology,Pediatrics, Perinatology and Child Health

Reference30 articles.

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Semen preparation techniques for intrauterine insemination. Cochrane Database Syst Rev. 2004;17(3):1-3. 7. Bui A, Sharma R, Henkel R, Agarwal A. Reactive oxygen species impact on sperm DNA and its role in male infertility. Andrologia. 2018;50(8):1-10. 8. White D, Phillips D, Bedford J. Factors affecting the acrosome reaction in human spermatozoa. J Reprod Fertil. 1990;90(1):71-80. 9. Jackson RE, Bormann CL, Hassun PA, Rocha AM, Motta EL, Serafini PC, et al. Effects of semen storage and separation techniques on sperm DNA fragmentation. Fertil Steril. 2010;94(7):2626-30. 10. Hosseini A, Khalili MA. Improvement of motility after culture of testicular spermatozoa: the effects of incubation timing and temperature. Transl Androl Urol. 2017;6(2):271. 11. Çok T, Aytaç PÇ, Şimşek E, Haydardedeoğlu B, Kalaycı H, Özdemir H, et al. The effect of preserving prepared sperm samples at room temperature or at 37 C before intrauterine insemination (IUI) on clinical pregnancy rate. Turk J Obstet Gynecol. 2015;12(1):6-10. 12. Matsuura R, Takeuchi T, Yoshida A. Preparation and incubation conditions affect the DNA integrity of ejaculated human spermatozoa. Asian J Androl. 2010;12(5):753–9. 13. Organization WH. WHO laboratory manual for the examination and processing of human semen. 2010. 14. Cohen J, Fehilly CB, Walters DE. Prolonged storage of human spermatozoa at room temperature or in a refrigerator. Fertil Steril. 1985;44(2):254-62. 15. Villarroya S, Scholler R. Lateral diffusion of a human sperm-head antigen during incubation in a capacitation medium and induction of the acrosome reaction in vitro. J Reprod Fertil. 1987;80(2):545-62. 16. Osheroff JE, Visconti PE, Valenzuela JP, Travis AJ, Alvarez J, Kopf GS. Regulation of human sperm capacitation by a cholesterol efflux-stimulated signal transduction pathway leading to protein kinase A-mediated up-regulation of protein tyrosine phosphorylation. Mol Hum Reprod. 1999;5(11):1017-26. 17. Peer S, Eltes F, Berkovitz A, Yehuda R, Itsykson P, Bartoov B. Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37° C? Fertil Steril. 2007;88(6):1589-94. 18. Aboulmaouahib S, Madkour A, Kaarouch I, Saadani B, Sefrioui O, Louanjli N, et al. Effect of semen preparation technique and its incubation on sperm quality in the Moroccan population. Andrologia. 2017;49(6):1-7. 19. Petrella C, Hsieh J, Blake E, Thrift K, Zacur H, Zhao Y. Human sperm can survive at room temperature for weeks: Measured by motility and viability of sperm maintained under various conditions. Fertil Steril. 2003;80(3):210. 20. Lachaud C, Tesarik J, Cañadas ML, Mendoza C. Apoptosis and necrosis in human ejaculated spermatozoa. Hum Reprod. 2004;19(3):607-10. 21. Yavas Y, Selub MR. Intrauterine insemination (IUI) pregnancy outcome is enhanced by shorter intervals from semen collection to sperm wash, from sperm wash to IUI time, and from semen collection to IUI time. Fertil Steril. 2004;82(6):1638-47. 22. Fauque P, Lehert P, Lamotte M, Bettahar-Lebugle K, Bailly A, Diligent C, et al. Clinical success of intrauterine insemination cycles is affected by the sperm preparation time. Fertil Steril. 2014;101(6):1618-23. 23. Jansen CH, Elisen MG, Leenstra CW, Kaaijk EM, van Stralen KJ, Verhoeve HR. Longer time interval between semen processing and intrauterine insemination does not affect pregnancy outcome. Fertil Steril. 2017;108(5):764-9. 24. Ahmed I, Abdelateef S, Laqqan M, Amor H, Abdel‐Lah M, Hammadeh M. Influence of extended incubation time on human sperm chromatin condensation, sperm DNA strand breaks and their effect on fertilisation rate. Andrologia. 2018;50(4):1-8. 25. Nabi A, Khalili M, Halvaei I, Roodbari F. Prolonged incubation of processed human spermatozoa will increase DNA fragmentation. Andrologia. 2014;46(4):374-9. 26. Aitken RJ. Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev. 1995;7(4):659-68. 27. De Lamirande E, Jiang H, Zini A, Kodama H, Gagnon C. Reactive oxygen species and sperm physiology. Rev Reprod. 1997;2(1):48-54. 28. Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil. 1987;81(2):459-69. 29. Tvrdá E, Arroyo F, Ďuračka M, López-Fernández C, Gosálvez J. Dynamic assessment of human sperm DNA damage II: the effect of sperm concentration adjustment during processing. J Assist Reprod Genet. 2019;36(4):799-807.

2. Lifestyle and fertility: the influence of stress and quality of life on male fertility

3. Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique

4. Effect of incubating human sperm at room temperature on capacitation-related events

5. Temperature controlled centrifugation improves sperm retrieval

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