Screening Studies of Chemical Composition, Antibacterial Activity and Acute Toxicity of Siberian Fir Oil Fraction

Author:

Kotova Tatiana1,Valnyukova Anastasia2,Fedorova Yulia2,Khudyntsev Konstantin3,Bhatia Naveen4,Zornichenko Grigory2

Affiliation:

1. Ural State University of Economics, Yekaterinburg, Russian Federation

2. Kemerovo State Medical University, Kemerovo, Russian Federation

3. LLC "Taezhnoye", Tomsk region, Semiluzhki village, Russian Federation

4. Hospital and Medical Research Centre PD Hinduja, Mumbai, India

Abstract

Siberian fir is a common species in the taiga zones. A man produces oils, balms, extracts, cell sap, phytoconcentrates, syrups, etc., used in the food, phar-maceutical, perfume and cosmetic industries, in the household chemicals produc-tion and in medicine from the waste left after harvesting the wood. Siberian fir waste processing products have antioxidant and anti-inflammatory properties and can be components of the biologically active additives and functional prod-ucts manufacture. The research aims at studying chemical composition of biologically active substances, macro- and microelements, as well as the antibacterial activity and toxicity of the oil fraction obtained from Siberian fir. The study ob-ject is the oil fraction obtained from the Siberian fir extract by extraction with liquid carbon dioxide. A man run screening and identification of target biologically active substances using methods of analytical HELC, IR spectroscopy (IR), gas-liquid chromatography-mass spectrometry (GLC-MS). The researchers de-termined antibacterial activity of the samples in relation to ten clinical microor-ganism isolates by the agar diffusion method. They studied toxicity on outbred mice, males and females CD-I at doses of 2.5, 1.25, 0.625 g/kg animal weight. The thesis consists of microbiological parameters of Siberian fir extract; experi-mental data on the qualitative and quantitative content of biologically active sub-stances, macro- and microelements in the oil fraction of Siberian fir. The study results of the oil fraction indicate that it is a rich source of biologically active sub-stances with antioxidant and anti-inflammatory properties; it belongs to the third hazard class (moderately dangerous substances). Microbiological indicators are within the permissible norm. The oil fraction of Siberian fir extract can be rec-ommended in the production of biologically active additives, functional products, but only after deep processing associated with the components fractionation of the raw materials used.

Publisher

Ural State University of Economics

Subject

Materials Chemistry,Economics and Econometrics,Media Technology,Forestry

Reference21 articles.

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