Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells

Author:

Cizkova Katerina1,Foltynkova Tereza1,Gachechiladze Mariam2,Tauber Zdenek1

Affiliation:

1. Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University

2. Department of Clinical and Molecular Pathology & Laboratory of Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University

Publisher

Japan Society of Histochemistry & Cytochemistry

Subject

Cell Biology,Histology,Physiology,Biochemistry,Pathology and Forensic Medicine

Reference22 articles.

1. 1 Aeffner, F., Wilson, K., Martin, N. T., Black, J. C., Hendriks, C. L. L., Bolon, B., et al. (2017) The Gold Standard Paradox in Digital Image Analysis: Manual Versus Automated Scoring as Ground Truth. Arch. Pathol. Lab. Med. 141; 1267–1275.

2. 2 Aeffner, F., Zarella, M. D., Buchbinder, N., Bui, M. M., Goodman, M. R., Hartman, D. J., et al. (2019) Introduction to Digital Image Analysis in Whole-slide Imaging: A White Paper from the Digital Pathology Association. J. Pathol. Inform. 10; 9–9.

3. 3 Bankhead, P., Loughrey, M. B., Fernández, J. A., Dombrowski, Y., McArt, D. G., Dunne, P. D., et al. (2017) QuPath: Open source software for digital pathology image analysis. Sci. Rep. 7; 16878.

4. 4 Bankhead, P., Fernández, J. A., McArt, D. G., Boyle, D. P., Li, G., Loughrey, M. B., et al. (2018) Integrated tumor identification and automated scoring minimizes pathologist involvement and provides new insights to key biomarkers in breast cancer. Lab. Invest. 98; 15–26.

5. 5 Bellemare, J., Rouleau, M., Harvey, M., Popa, I., Pelletier, G., Têtu, B., et al. (2011) Immunohistochemical expression of conjugating UGT1A-derived isoforms in normal and tumoral drug-metabolizing tissues in humans. J. Pathol. 223; 425–435.

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