Studies and Enhancement of Natural Bioactive Compounds Content through Media Optimization

Abstract

In these studies, endophyte bacteria were isolated from a medicinal plant root of Adhathoda beddomei (adosa). The antimicrobial activity was demonstrated against different pathogenic and nonpathogenic bacteria. Identification of endophytes was done based on external morphological characteristics with the help of a Scanning Electron Microscope (SEM). another challenging problem in this regard is that the efficiency of the endophytic bacteria to produce bioactive compounds is not as high as expected because, in many previous works in literature, it has been reported that yields are generally meagre. Through this study, we have tried to enhance the production of bioactive metabolites using media optimization and Resonance Surface Methodology (RSM). These are generally from µg/l to less than mg/l and are therefore not yet appropriate for fermentative production on an industrial level.