An <i>In Vitro</i> Study for Evaluating the Antimicrobial Efficacy of Polyherbal Combination Against <i>Escherichia coli</i> in the Management of <i>Mutrakrichra</i> (Urinary Tract Infection)

Abstract

Millions of people worldwide experience Urinary Tract Infections (UTIs), which rank among the most widespread bacterial infections. UTIs are a common health issue, particularly among women and certain populations. Escherichia coli is the most frequent pathogen, causing 75-90 % of UTIs. Timely diagnosis, appropriate treatment, and preventive measures can help reduce the burden of UTIs and minimize complications associated with these infections. In order to address this issue, classical formulations are mentioned in Ayurvedic texts. In the current study, hydroalcoholic extracts of Emblica officinalis (Amalaki) and Berberis aristata (Daruharidra) were prepared. The antimicrobial activity of the extracts and their combination was evaluated by establishing their Minimum Inhibitory Concentration (MIC) and measuring the zone of inhibition. The combination of Amalaki and Daruharidra with honey shows significant inhibition of E. coli which is comparable with tablet Norfloxacin. This study suggests that Emblica officinalis (Amalaki) and Berberis aristata (Daruharidra) can be used in Mutrakrichra associated with Mutradaha in the context of urinary tract infections.