Development and Validation of HPLC and UV Spectrophotometric Method for the Quantification of Cinnamaldehyde in Cinnamon Bark Extract

Abstract

Cinnamaldehyde is the prime constituent of cinnamon bark and cassia oil. It is used as a flavoring agent. Numerous methods described the determination of cinnamaldehyde based on volumetric analysis, ultraviolet spectrometry, fluorimetry, thin layer chromatography, liquid chromatography and gas chromatography. The natural absorbance of cinnamaldehyde is at 286 nm which is used as a base for its determination through spectrophotometry or a suitable derivatizing reagent is used for its estimation. Most of these methods were simultaneous estimation methods and if non-simultaneous than not sensitive. Therefore, in the present study, sensitive HPLC and UV Spectrophotometric procedures have been established for the estimation of cinnamaldehyde in Cinnamon extract. The retention time of cinnamaldehyde was 7.21 minutes and absorption maxima come out to be 282 nm. 2.55 ± 0.003 mg/ml quantity of cinnamaldehyde was present in the cinnamon extract which is detected by UV Spectrophotometric method. Accuracy information appeared in the range that gives decent recovery figures for both processes. Sensitivity data furnished LOD 0.062 μg/ml and LOQ 0.19 μg/ml for HPLC and LOD 0.104 μg/ml and LOQ 0.312 μg/ml for UV Spectrophotometric method. The developed methods were found to be rugged and robust. The repeatability, Inter-day,and Intra-day precision of cinnamaldehyde provided RSD below 2% presenting the planned process to be extremely specific. Various factors to validate HPLC and UV Spectrophotometric methods of cinnamaldehyde were estimated and both methods show no significant difference. Developed procedures were statistically checked as per ICH guidelines.