Screening of Neuroprotective Action of Hydroethanolic Extract of Leaf of <i>Clematis buchananiana</i> in Diabetic-Induced Neuropathy

Abstract

Diabetic neuropathy is the most dangerous complication of diabetes which is very difficult to treat. Diagnosis in the early stage prevents worse outcomes of the illness. Determine the pharmacological effect of hydroethanolic extract of Clematis buchananiana leaves was investigated and screened for determination of neuroprotective effect in diabetic-induced neuropathy using Wistar rats. Streptozotocin normally induces diabetes within 3 days. The destruction of the beta cells present in the pancreatic gland leads to diabetes. The confirmation of diabetes was done with the help of a glucometer. The experimental models used for the assessment of analgesic activity in Wistar albino rats included the tail immersion method and acetic acid-induced writhing method. After completion of the research study, it was found that the animals treated with standard drug (Gabapentin, 100 mg/kg) had maximum analgesic action, followed by a higher dose of hydroethanolic extract of Clematis buchananiana leaf (HEELCB, 400 mg/kg). The major observation was that hydroethanolic extract of Clematis buchananiana has significant analgesic action and it validates the traditional claim of the plant as an analgesic agent.