Identification of bio-active compounds in indigenous <i>Trichoderma asperellem</i> against <i>Fusarium oxysporum</i> f. sp. <i>ciceris:</i> A causal agent of chickpea wilt

Abstract

Wilt caused by Fusarium oxysporum f. sp. ciceris (Padwick) is an important soil-borne disease and considered as most serious and widespread disease of chickpea throughout the world. Twenty indigenous isolates of Trichoderma spp. were isolated from the rhizosphere of chickpea in different geographic regions of Karnataka. Among twenty isolates, a higher concentration of volatile compounds was produced by TR-14 (72.22%) followed by TR-19 (66.67%) and TR-9 (65.93%). Further, the efficient strain that is TR-14 was identified as Trichoderma asperellum molecularly and was used to extract metabolites by using solvent extraction technique and subjected to GC-MS/MS analysis. The results have shown presence of twenty-nine compounds at different retention times ranging from 4.910 to 21.868 min. and the mass-to-charge (m/z) ratio from 43 to 190. Out of twenty-nine compounds, the concentration of 2-Imidazol-1-ylmethyl-pyridine 1-oxide (C9H9N3O) was highest with the maximum area (14375440) and retention time (19.842). Apart from this compound, 7-Isopropylidene-5-methyl-2, 3-diazabicyclo (2.2.1) hept-5-ene-2,3 dicarboxylic acid, diethyl ester was present at the highest peak with a retention time of 20.021.