Author:
Kaur Navjot,Sharma M.,Lonare M. K.,Udehiya R.,Singh D.
Abstract
The present investigation was planned to evaluate the effect of curcumin (CUR) and resveratrol (RES) on oxidative stress indicators (LPO, O2- radical, ROS and TPC), antioxidant status (GSH, GPx, SOD, CAT and GST) and cytotoxicity markers (ALP, LDH, GGT and CK-MB) in mesenchymal stem cells (MSCs) derived from buffalo bone marrow during the freeze-thaw cycle of one month. MSCs were isolated and cultured in high glucose DMEM supplemented with 15% FBS. MSCs were found to be positive for alkaline phosphatase (AP) activity and stem cell markers (CD73 and OCT4). Results revealed a significant reduction in (p≤0.05) in oxidative stress parameters and cytotoxicity markers, while, the levels of cellular antioxidants were found to be significantly increased (p≤0.05) in CUR/RES alone and in combination groups as compared to the control group. In conclusion, the addition of bioantioxidants in the cryopreservation medium has improved the post-thaw cell recovery through suppression of oxidative stress-induced during the freeze-thaw cycle.
Publisher
Informatics Publishing Limited
Subject
Health, Toxicology and Mutagenesis,Toxicology,Health, Toxicology and Mutagenesis,Toxicology
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