Author:
Vineela Sathuluri,Manga Devi Chinta,Santhrani Thakur
Abstract
Acrylamide (ACR) is a potent neurotoxic to humans and animals. Neuroprotective effect of naringin was evaluated on ACR induced cytotoxicity using U87MG cells as in-vitro model and rat as in vivo model. ACR (50 mg/kg, i.p.) and Naringin (50 & 100 mg/kg) were administered to rats for 4 weeks. After 4 weeks, rats were sacrificed and sciatic nerves were isolated to determine the biochemical and apoptotic parameters. The exposure of U87MG cells to ACR reduced cell viability. Pretreatment of cells with 5–300 μg/ml naringin before ACR treatment signiï¬cantly attenuated ACR cytotoxicity in a dose-dependent manner. Naringin down-regulated the Bax and up-regulated the Bcl2 protein expression levels and also scavenged ACR induced free radicals in rats. In conclusion, our results indicated that pretreatment with naringin protected cells and rats from ACR-induced cytotoxicity and the neuroprotective effect was due to its antiapoptotic and antioxidant potential.
Publisher
Informatics Publishing Limited
Subject
Health, Toxicology and Mutagenesis,Toxicology,Health, Toxicology and Mutagenesis,Toxicology
Reference47 articles.
1. Zyzelewicz D, Nebesny E, Oracz J. Acrylamide-formation, physicochemical and biological properties. Bromatol Chem Toksykol. 2010; 43:415–27.
2. Jankowska JHJ, Potocki A. Acrylamide as a foreign substance in food (in Polish). Problemy Higieny I Epidemiologii. 2009; 90:171–4.
3. Dybing E, Sanner T. Risk assessment of acrylamide in foods. Toxicol Sci. 2003; 75:7–15. https://doi.org/10.1093/toxsci/kfg165. PMid:12805639
4. Grob K, Biedermann M, Biedermann-Brem S, Noti A, et al. French fries with less than 100 μg/kg acrylamide. A collaboration between cooks and analysts. Eur Food Res Technol. 2003; 217:185–194. https://doi. org/10.1007/s00217-003-0753-9
5. Cummins E, Butler F, Brunton N, Gormley R. Factors affecting acrylamide formation in processed potato products a simulation approach. 13th World Congress of Food Science & Technology 2006; Nantes: 719. https://doi.org/10.1051/IUFoST:20060719
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献