Breeding evaluation of the common buckwheat accessions (<i>Fagopyrum esculentum</i> Moench) obtained with the use of copper and zinc ions

Abstract

Relevance. Employing new biotechnological methods in the breeding of buckwheat, including the screening of forms with tolerance to copper and zinc ions, might increase the adaptability of genotypes and allow the creation of promising starting material for further breeding.Methods. The research on common buckwheat with tolerance to copper and zinc ions and breeding evaluation of the obtained accessions were conducted at FSBSI «FSC of Agricultural Biotechnology of the Far East named after A.K. Chaiki» in 2020–2023. The research object was 10 accessions obtained from buckwheat varieties Kitavasesoba (Japan) and Izumrud (Russia) and hybrid Izumrud×Kitavasesoba, whose microshoots and seeds were exposed to copper and zinc ions. Released variety Izumrud was used as the standard. The method of cell and tissue culture in vitro as well as the germination of seeds in concentrated solutions of copper and zinc salts in vivo were employed.Results. In the conditions of the monsoon climate of Primorsky Krai, a three-year test of 10 buckwheat samples obtained under the influence of copper and zinc ions in culture in vitro and in vivo was carried out. As a result of the study, three samples were selected for further selection (No. 989, 1013, 783), which are characterized by a complex of economically valuable traits: high seed productivity (6.5–7.1 g), stress resistance, genetic flexibility, high mass of 1000 seeds (37.8–44.8 g), a combination of high mass of 1000 kernels (27.4–33.1 d) with low film content (23.3–25.5%), and two samples (No. 997, 1005) with an increased content of rutin in fruits (7.1–7.7 mg / 100 g).