Application of the PCR method to detect the falsification of sheep milk with goat milk

Abstract

The article discusses the option of using PCR analysis to assess the purity of the species composition of heat-treated sheep's milk. In the course of the work, the genetic material of the main dairy breeds of goats and sheep represented on the territory of Russia was studied. For this purpose, the 12S rRNA of a goat and a sheep was used as a target gene, on the basis of which a pair of primers 12SCH-DIR (5'-AAACGTGTTAAAGCACTACATC-3 ‘) and 12SCH-INV (5'-GTCTTAGCTATAGTGTATCAGCTG CA-3') for goat and 12SM-FW (5'-CTAGAG-GAGCCTGTTCTATAATCGATAA-3 ‘) and 12SOA-INV (5'-GTCTCCTCTCG TGTGGTTGAGATA-3') for sheep. The resulting primers were tested for specificity by analyzing the milk of various breeds of goats (Saanen, Toggenburg, Alpine and Nubian dairy) and sheep (Tsigai, Assaf and East Friesian). As a result of the study of binary mixtures of sheep and goat milk, it was found that the use of this method of PCR analysis makes it possible to detect impurities of goat's milk in sheep's milk at a level of 0.1%. It has also been shown that the technique is applicable to both raw milk and processed milk.