The effect of a preparation based on secondary bile acids on the regeneration of the liver parenchyma in the modeling of toxic hepatitis

Abstract

Relevance. When using rodents as biological models using hepatotoxicants of different structure and mechanism, it was noted that tissue repair plays a decisive role in determining the final result of toxicity. Tissue regeneration is a complex process driven by multilevel cellular signaling involving a number of chemokines, cytokines, growth factors and nuclear receptors leading to the expression of promitogenic genes and cell division. Tissue repair also includes regeneration of the liver’s extracellular matrix and angiogenesis, processes required to completely restore the structure and function of the liver. Stimulating liver tissue through pharmacological correction is critical for recovery from toxic damage.Methods. The study was conducted on the basis of the St. Petersburg State University of Veterinary Medicine for 21 days. Two groups of white nonlinear rats, nine animals in each group, were used in the experiment. When modeling toxic hepatitis and further pharmacological correction promoting the regeneration of the liver parenchyma the drug “Hepaton” was used based on secondary bile acids (therapeutic dosage for rats – 0.5 mg/kg, duration of pharmacological correction – 21 days).Results. After 21 days, the experimental animals were euthanized according to the principles of bioethics, after which a histological examination of the liver was carried out. On histological sections of the liver of animals of the second experimental group the drug “Hepaton” reliably positively influenced the regenerative abilities of the liver parenchyma. Based on both experimental and data from the scientific literature, it can be concluded that drugs based on bile acids have an extremely wide range of applications in the future for pharmacological correction of various pathologies of the hepatobiliary system.