Use of MONTANIDE ISA 78 VG as adjuvant for the manufacture of antibacterial vaccines for chickens

Abstract

Relevance. The use of antimicrobial agents and vaccine prophylaxis are the main ways to prevent and control most diseases of bacterial etiology. At the same time, uncontrolled use of antimicrobial agents without determining the sensitivity of microorganisms, as a rule, does not give the opportunity to obtain a positive therapeutic result. At the same time, a competently designed scheme of inactivated vaccine administration, taking into account the epizootic situation, is an effective and safe tool for controlling bacterial diseases.In this regard, the results of testing of vaccine samples against bacterial diseases of birds based on the modernized oil adjuvant Montanide ISA 78 VG presented in this article are interesting and modern.Methods. Four vaccine samples were manufactured for research based on the oil adjuvant Montanide ISA 78 VG. The first sample of the vaccine is against avian salmonellosis, the second — against avian pasteurellosis, the third — against avian respiratory mycoplasmosis, the fourth — against avian hemophilosis. All manufactured vaccine samples were investigated for sterility, stability and viscosity of the emulsion according to generally accepted methods. To determine the reactogenicity and antigenic activity of the vaccine samples, young egg-laying hens aged 25–40 days were used.Results. Analysis of the obtained results of physical and biological properties of tested inactivated vaccines against bacterial diseases of birds allows us to conclude that the vaccine against respiratory mycoplasmosis of birds made on the basis of adjuvant Montanide ISA 78 VG is a safe and effective immunobiological preparation.