IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF THE FRUIT EXTRACT OF RIVINA HUMILIS L. (RED PIGEON BERRY)

Abstract

The purpose of this study was to evaluate the cytotoxic and antioxidant properties of a hydromethanolic extract of Rivina humilis L fruits extract (HMERH) on human cancer cell lines and various in vitro models. HMERH concentrations ranging from 5 to 400mg/ml were utilized in several assay methods such as hydroxyl radicals, DPPH, superoxide radical scavenging activity, metal chelating, and nitric oxide radical test, ABTS, and reducing power assay. All antioxidant assays employed ascorbic acid as the standard. Its short-term cytotoxicity on EAC and DLA cells was determined using the trypan blue dye exclusion method, as well as the lactate dehydrogenase (LDH) leakage assay and in vitro cytotoxicity on MEF-L929, DU-145, and PC-3 cells using the MTT assay. The total antioxidant potential was also determined using the phosphomolybdenum test. HMERH demonstrated considerable cytotoxicity against all cancer cell lines examined. The findings of the free radical scavenging activities demonstrated a concentration-dependent and antiradical activity caused by the reduction of ABTS, DPPH, NO, OH, and SO radicals to non-radical form. The findings reveal that antioxidants and cytotoxic agents have potent antioxidant and cytotoxic effects on cancer cell lines. Hence, this fruit extract has the potential to be a natural antioxidant and anticancer drug source, which could be valuable in the development of new anticancer therapies.