NEUROPROTECTIVE EFFECT OF VANDA TESSELLATA AS “RASNA” SPECIES, ON ALUMINIUM CHLORIDE INDUCED ALZHEIMER’S IN RATS

Abstract

The present study focused on evaluation of neuroprotective effect of hydromethanolic extracts of Vanda tessellate (VT), also considered as Rasna. Aluminum Chloride (AlCl3) induces neuroinflammation in rats and finally the development of AD. PASS online and molecular docking insilico studies were conducted with PPAR-γ for β-sitosterol and AChE for gigantiol. Total 36 trained Wistar rats were divided into VI groups 6 in each. Group I - normal control, Group II – Disease control, Group III – Rivastigmine (0.3mg/kg, p.o), Group IV and V – Hydromethanolic extract of VT (HMEVT, 150mg/kg, 300mg/kg, p.o) respectively, Group VI - Ayurvedic Formulation of Rasna (AFR) (1ml/kg, p.o). All the animals received Aluminum Chloride (AlCl3) (300mg/kg, p.o) except group I. The rats were treated for 20 days but mean time behavioural study, body weight changes were monitored on 0th, 7th, 14th& 20th day. On 21th day, rats were sacrificed, brains were isolated, then antioxidant enzymes levels, protein content and neurotransmitters levels were determined. Histopathology of cortex and hippocampus  parts of the brain were studied. Group II animals showed reduction in locomotor activity, increased in the number of entries as well as time spent in closed arm and time taken to climb the pole was increased but it was reversed in groups treated with 150mg/kg, 300mg/kg doses of HMEVT and AFR. Increased level of protein content, malondialdehyde, reduction in body weight and antioxidants enzymes like superoxide dismutase, catalase, glutathione were observed in disease control group and it was due to free radicals generation and were corrected and restored in groups treated with HMEVT and AFR. Moreover, the histopathological report also showed cellular level protection efficacy found with HMEVT and AFR. The neuroprotective action of HMEVT was due to the active constituents and was proved in insilico study. The order of neuroprotective efficacy was HMEVT > AFR.