Abstract
The objective of the present study was to evaluate the influence of the genotypes of two single nucleotide polymorphisms (SNPs) – c.78C>T located in the growth hormone gene (GH) and c.106C>G in the growth hormone receptor gene (GHR) on individual body weight (IBW) during the growing period at 35, 70 and 90 d of age on a total of 107 weaned Californian breed rabbits. The restriction fragments obtained revealed that 74.8% of the rabbits carrying c.78C>T SNP and 52.3% of the rabbits carrying c.106C>G SNP were heterozygous, which indicated a moderate level of genetic diversity in this Californian population. Association analysis based on a single-gene approach revealed that c.78C>T polymorphism in the GH gene had a significant effect (P<0.05) on the weight at 70 and 90 d of age. The highest IBW (2530.4±66.6 g) was observed in rabbits carrying the c.78C>T TT genotype, and detected individuals were significantly affected by the dominance effect. Significant differences were observed between individuals with homozygous c.106C>G CC genotype and those with heterozygous CG genotype. The highest IBW (2462.0±198.3 g) was observed in rabbits carrying the c.106C>G CC genotype and detected individuals were significantly affected by the additive effect. A total of nine combined genotypes of c.78C>T and c.106C>G SNPs was found in the study, of which only four major groups (CT/CC, CC/CG, CT/CG, and CT/GG) were concerned in the diplotype analysis. Significant differences were observed between individuals with CT/CC and CC/CG genotype combinations, and between those with the CC/CG and CT/GG diplotypes. However, the highest IBW at 90 d of age (2447.2±213.8 g) was observed in rabbits carrying the CT/CC genotype combinations. The highest coefficient of determination found for individual body weight at 90 d of age (R2=10.8%) indicated a high effect of genotype combinations. In conclusion, the results obtained suggested that c.78C>T of GH gene and c.106C>G of GHR gene could be useful candidate genes to improve growth performance in Californian rabbits with potential application in rabbit breeding programmes.
Publisher
Universitat Politecnica de Valencia
Subject
Animal Science and Zoology
Reference33 articles.
1. Bai W.L., Zhou C.Y., Ren Y., Yin R.H., Jiang W.Q., Zhao S.J., Zhang S.C., Zhang B.L., Luo G.B., Zhao Z.H. 2011. Characterization of the GHR gene genetic variation in Chinese indigenous goat breeds. Mol Biol Rep., 38: 471-479. https://doi.org/10.1007/s11033-010-0130-2
2. Baselga M. 2004. Genetic improvement of meat rabbits. Programmes and diffusion. In Proc.: 8th World Rabbit Congress, September 7-10, 2004, Pueblo, Mexico, 1-13.
3. Dekkers J.C. 2004. Commercial application of marker- and geneassisted selection in livestock: Strategies and lessons, J. Anim. Sci., 82, 13: 313-328.
4. Deng X.S., Wan J., Chen S.Y., Wang Y., Lai S.J., Jiang M.S., Xu M. 2008. The correlations between polymorphism of growth hormone receptor gene and butcher traits in rabbit. Yi chuan. Hereditas, 30: 1427-1432. https://doi.org/10.3724/SP.J.1005.2008.01427
5. Dimitrova I., Dimitrov T., Teneva A., Tzvetkova H. 2008. Rabbit production in Bulgaria. Biotech. Anim. Husbandry, 24: 1-2: 149-154. https://doi.org/10.2298/BAH0802149D
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献