Affiliation:
1. State Scientific Institution "Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus"
Abstract
A comparative analysis of the effectiveness of organogenesis from the callus cells of hypocotyl segment explants in rapeseed varieties Leader and Magnat regionalized in Belarus was carried out. It was established that the Leader, compared to the Magnat variety, is more suitable for organogenesis from plant explants in vitro. It was shown that for the Leader variety the regeneration efficiency is up to 10 plants adapted ex vitro per explant, when using a method, according to which hypocotyl explants are first exposed to auxin (2,4-dichlorophenoxyacetic acid, 1 mg/l), and then to cytokinin (6-benzylaminopurine, 5–8 mg/l).
Publisher
National Academy of Sciences of Belarus