CONSTRUCTION OF A BACTERIAL STRAIN FORMING INCLUSION BODIES, EXHIBITING DIADENYLATE CYCLASE ACTIVITY

Abstract

Using Recombinant DNA Technology, the novel bacterial recombinant strain Escherichia coli DAC-22, a source of diadenylate cyclase that catalyzes the transformation of adenosine-5′-triphosphate into cyclic 3′,5′-diadenylate (cyclo-di-AMP), was developed. The strain was derived by the transformation of E. coli Rosetta (DE3) pLysS cells with the recombinant plasmid pET42a+ wherein the disA gene responsible for the synthesis of the diadenylate cyclase of Bacillus thuringiensis was inserted. The producing capacity of the new strain with respect to diadenylate cyclase localized in catalytically active inclusion bodies equaled 720 units per liter of liquid culture. The newly engineered strain is destined for use in the technology related to the production of pharmaceutically promising cyclo-di-AMP.