POLYMORPHISM OF ALLELES ASSOCIATED WITH LOW TEMPERATURE RESISTANCE IN REPRESENTATIVES OF THE BRASSICACEACE FAMILY WITH A WIDE DISTRIBUTION AREA

Abstract

The polymorphism of loci associated with cold resistance in representatives of the Brassicaceae family was studied. We used 11 SSR markers significantly correlated with relative electrical conductivity of tissue and MDA content, 4 SCAR-markers linked to the alleles of gene CCA1, as well as SSR Ol10 and SSR BnOl10, closely linked to QTLs associated with plant flowering start time, frost resistance, and winter survival. Verification of samples using the BoCCA1-1R1 SCAR marker showed that the BoCCA1-1 allele, which is characteristic of cold resistant varieties, is contained in the genomes of 26 out of 88 analyzed forms, including the Belarusian varieties of rapeseed Zenit, Dobrodey, Martyn. Work has begun on the determination of CNV for SSR markers associated with MDA content and relative electrical conductivity of tissues in representatives of the Brassicaceae family. It was found that the DNA markers BrGMS102 and BrGMS4511, which are not effective for detecting polymorphism at genetic loci associated with cold resistance in representatives of the Brassicaceae family, show less than one copy per genome. The effective marker BnGMs180-1 showed variability: from two to twelve copies per genome. Consequently, gene copy number has an effect on increasing resistance to low temperatures in representatives of the Brassicaceae family.