STUDYING OF THE STS-MARKERS POLYMORPHISM IN HETEROBASIDION ISOLATES INFECTING NORWAY SPRUCE (PICEA ABIES (L.) H.KARST.) IN BELARUS

Author:

Nestyuk A. M.1,Panteleev S. V.2,Yarmolovich V. A.1,Baranov O. Yu.3

Affiliation:

1. Belarusian State Technological University

2. Institute of Forests, National Academy of Sciences of Belarus

3. Belarusian State Technological University; Department of Biological Sciences of the National Academy of Sciences of Belarus

Abstract

The article presents the results of studying the genetic polymorphism of Heterobasidion (root rot agent) isolates that infect Norway spruce (Picea abies) in various regions of Belarus. Based on the data obtained, a comparative assessment of the suitability of STS markers for species diagnostics of fungal isolates (Heterobasidion annosum (Fr.) Bref. sensu stricto (s. s.) and Heterobasidion parviporum Niemelä & Korhonen) was carried out. Samples of the sporocarp and infected wood were collected in Norway spruce stands on the territory of three geobotanical subzones — in seven forestry enterprises of the Ministry of Forestry of the Republic of Belarus, on the territory of the Negorelsky educational and experimental forestry and the National Park "Belovezhskaya Pushcha". Based on the comprehensive analysis of the samples, markers for specific molecular genetic diagnosis of H. annosum and H. parviporum were recommended.

Publisher

National Academy of Sciences of Belarus

Reference25 articles.

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2. Mitchelson, K. Diagnosis and differentiation of intersterility groups / K. Mitchelson, K. Korhonen // Heterobasidion annosum: ecology, biology, impact and control / edited by S. Woodward [et al.]. – Cambrige, 1998. – Ch. 5. – P. 71–92.

3. Федоров, Н. И. Ферментативная активность разных штаммов корневой губки / Н. И. Федоров, Н. И. Стайченко, Л. М. Неустроева // Микология и фитопатология. – 1975. – Т. 9, № 3. – С. 235–239.

4. Korhonen, K. Intersteriity groups of Heterobasidion annosum / K. Korhonen // Commun. Inst. For. Fenn. – 1978. – Vol. 94, № 6. – 25 p.

5. Otrosina, W. J. Heterobasidion occidentale sp. nov. and Heterobasidion irregulare nom. nov.: a disposition of North American Heterobasidion biological species / W. J. Otrosina, M. Garbelotto // Fungal Biol. – 2010. – Vol. 114. – P. 16–25.

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