IDENTIFICATION OF POLYMORPHISMS 1843C>T IN THE RYR1 GENE AND 5860C>T IN THE DMD GENE ASSOCIATED WITH THE STRESS SINDROM OF PIGS USING KASP TECHNOLOGY-Reference-Cited by-同舟云学术

IDENTIFICATION OF POLYMORPHISMS 1843C>T IN THE RYR1 GENE AND 5860C>T IN THE DMD GENE ASSOCIATED WITH THE STRESS SINDROM OF PIGS USING KASP TECHNOLOGY

Published:2021-04-12 Issue: Volume:30 Page:61-67
ISSN:1999-9127
Container-title:Молекулярная и прикладная генетика
language:
Short-container-title:Молекулярная и прикладная генетика

Author:

Ramanishka E. L.¹,Mikhailova M. E.¹,Sheyko R. I.¹

Affiliation:

1. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus

Abstract

Pig exposure to stress is a serious economic problem for pig breeding. We have studied genetic factors that determine the sensitivity of pigs to stress in different breeds of pig such as polymorphisms 1843C>T in the RYR1 gene (rs344435545) and 5860C>T in the DMD gene (rs196952080) in the Republic of Belarus using the competitive allelespecific PCR (KASP) method. The frequency of occurrence of animals carrying stress syndrome associated with the DMD gene polymorphism (TO/TT genotype) was 2.8% in the Landrace breed. The mutant T-allele was identified in the sample of animals of the Landrace breed with a frequency of 14.2%, in Duroc – 1.3%, in Yorkshire – 0.8%. Mutant n-alleles were not found in the RYR1 gene in the studied sample of animals. The study results demonstrate that DNA diagnostics of breeding animals is required to identify nonsynonymous substitutions in RYR1 and DMD genes in order to control and eliminate stress syndrome in pig population.

Publisher

National Academy of Sciences of Belarus

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