ASSESSMENT AND APPLICATION OF MOLECULAR MARKERS IN BREEDING FOR THE RESISTANCE OF TOMATO (SOLANUM LYCOPERSICUM L.) TO LATE BLIGHT (PHYTOPHTHORA INFESTANS)-Reference-Cited by-同舟云学术

ASSESSMENT AND APPLICATION OF MOLECULAR MARKERS IN BREEDING FOR THE RESISTANCE OF TOMATO (SOLANUM LYCOPERSICUM L.) TO LATE BLIGHT (PHYTOPHTHORA INFESTANS)

Published:2021-12-08 Issue: Volume:31 Page:22-30
ISSN:1999-9127
Container-title:Молекулярная и прикладная генетика
language:
Short-container-title:Молекулярная и прикладная генетика

Author:

Babak O. G.¹,Drozd E. V.¹,Nekrashevich N. A.¹,Anisimova N. V.¹,Yatsevich K. K.¹,Bayeva I. E.²,Frantsuzionak A. V.²,Pugachova I. G.²,Dobrodkin M. M.²,Kilchevsky A. V.¹

Affiliation:

1. State Scientific Institution “Institute of Genetics and Cytology of the National Academy of Sciences of Belarus”

2. Belarusian State Agricultural Academy

Abstract

In order to use marker-assisted selection (MAS) methods in the development of hybrids and tomato varieties resistant to late blight, the efficiency of molecular markers to the alleles resistant to Phytophthora infestans known in the literature was assessed. High efficiency in the identification of homozygous and heterozygous forms using CAPS dTG63 (Hinf1) and SCAR NCLB-9-6678 markers to Ph2 and Ph3 alleles was established respectively. DNA extraction techniques, reaction mixture compositions, as well as amplification and visualization result modes were adapted. The presence of the Ph3 allele in F1-standard Adapt and Alamina hybrids, and F1 Azart, Agenchik, Dzivosny, and Black Brilliant hybrids included in the Register of Varieties of the Republic of Belarus, as well as in the breeding lines of the collections possessed by the Institute of Genetics and Cytology, NAS of Belarus, and the Belarusian State Agricultural Academy was demonstrated.

Publisher

National Academy of Sciences of Belarus

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