GENETIC TYPING OF LEUCONOSTOC MESENTEROIDES USING PCR-Reference-Cited by-同舟云学术

GENETIC TYPING OF LEUCONOSTOC MESENTEROIDES USING PCR

Published:2020-12-14 Issue:14 Volume: Page:40-49
ISSN:2220-8755
Container-title:Topical issues of processing of meat and milk raw materials
language:
Short-container-title:Aktual. vopr. pererab. mâsn. moločn. syrʹâ

Author:

Biruk A.¹,Tarashkevich Y.¹,Furik N.¹

Affiliation:

1. Institute for Meat and Dairy Industry

Abstract

We studied the possibility of using RAPD-PCR with primers: ERIC1R-1, ERIC2-1, BOXA1R, BOXA2R and Rep-PCR with primers P15, P16, XD8, XD9, RAPD-mes, (GTG)5 to identify genetic heterogeneity of 9 strains and 8 isolates of Leuconostoc mesenteroides. Three clusters of cultures with a high level of bootstrap support were identified as a result of phylogenetic analysis obtained when typing Leuconostoc. The obtained results indicate the possibility of revealing genetic differences in the profile of the generated amplicons among Leuconostoc mesenteroides strains using the combined methods of Rep-PCR and RAPD-PCR.

Publisher

National Academy of Sciences of Belarus

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